Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Red Fluorescence*

Catalogue Number: 22797-AAT

Manufacturer:AAT Bioquest
Type:Apoptosis Detection Kit
Shipping Condition:Blue Ice
Unit(s): 100 Tests

Description

Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for cell apoptosis since the activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses Z-DEVD-ProRed™ as the fluorogenic indicator for caspase-3 activity. Cleavage of ProRed™ DEVD blocking peptide residue by caspase 3 generates strongly red fluorescent ProRed™ that is monitored fluorimetrically at ~620 nm with excitation of ~530 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays. Using 100 ul of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 100 assays. Using 25 ul of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 400 assays.

Additional Text

Storage Note

Multiple;|6 months