Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit *Green Fluorescence*
Catalogue Number: 22849-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 6 months |
| Type: | Apoptosis Detection Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 Tests |
Description
Description: DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are secondarily labeled with a marker. All the existing TUNEL assays contain the highly toxic sodium cacodylate which might induces apoptosis and also decrease DNA production and DNA strands. Our Cell Meter™ TUNEL Apoptosis Assay Kit uses a proprietary buffer system free of sodium cacodylate. The kit is based on the incorporation of our proprietary fluorescent dye into the DNA fragments that form during apoptosis. The assay is optimized for the direct detection of apoptosis in either detached or attached cells without using any antibodies. The kit provides all the essential components with an optimized assay protocol. It is suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected at the popular FITC channel.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|6 months
22849 MSDS
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations