Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Deep Red Fluorescence*
Catalogue Number: 22855-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 6 months |
| Type: | Apoptosis Detection Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 tests |
Description
Description: Cell Meter™ TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation. The assay is non-radioactive and rapid. The TUNEL assay uses the terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of TF5-dUTP at the free 3'-hydroxyl ends of the fragmented DNAs. The resulted TF5-labeled DNAs are analyzed by fluorescence microscopy (Cy5 filter set) or flow cytometry with 633 or 640 nm laser with 660/20 nm filter (APC channel). Its red emission can be conveniently multiplexed with GFP labelled targets. Direct incorporation of fluorescent TF5-labeled nucleotides significantly reduces the number of test steps. The kit is optimized to detect apoptosis in fixed cells and formalin-fixed, paraffin-embedded tissue sections.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|6 months
22855 Protocol
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