mFluor™ Violet 500-Wheat Germ Agglutinin (WGA) Conjugate
Catalogue Number: 25452-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Protein - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: Wheat germ agglutinin (WGA) is a well-studied lectin known for its binding affinity to N-acetyl-D-glucosamine and sialic acid, making it a valuable tool in various biological applications. Its interaction with glycoconjugates enables widespread use of WGA derivatives and conjugates for fluorescence imaging and analysis, facilitating the labeling of yeast bud scars, fibrotic scar tissue, and the cell membranes of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer contains two identical, non-interacting binding sites complementary to 3 or 4 β-1,4-GlcNAc units. Among the monosaccharides tested, only GlcNAc shows strong binding to WGA, while ManNAc demonstrates no binding, and GalNAc exhibits weak binding. The mFluor™ Violet 500 labeled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 501 nm. Notably, the mFluor™ Violet 500 WGA conjugate retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis of various scientific investigations.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
25452 Protocol
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