ReadiUse™ Preactivated PE-iFluor® 700 Tandem
Catalogue Number: 2585-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Fluorophores |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
Description
Description: PE-Alexa Fluor® 700 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 720 nm. AAT Bioquest offers this preactivated PE-iFluor 700™ as a superior replacement to the popular PE- Alexa Fluor® 700 tandem. Compared to PE-Alexa Fluor® 700 tandem, PE-iFluor 700 is brighter with better FRET efficiency. It is used to facilitate the PE-Alexa Fluor® 700 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-iFluor 700 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based PE-Alexa Fluor® 700 tandem conjugation chemistry with better flow cytometry performance. In addition, our preactivated PE-iFluor 700 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Additional Text
Storage Note
Multiple;|6 months
2585 Protocol
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