Human COX-2 Assay Kit - IBL

Catalogue Number: 27186-IBL

Manufacturer:IBL - (Immuno-Biologicals Laboratories Co Ltd)
Type:Human ELISA Kit
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 96 Well
Range: 1.09 ~ 70 ng/mL
Sample type: Cell extract
Sample size:


Description: The kit # 27186 has improved stability and convenience from the former kit # 17186. The improved kit has some advantages as follows. 1) We have changed the formulation of HRP conjugated antibody from lyophilized product to liquid (0.4mL X 1). By this change, the stability of the conjugate has improved so that customers can be use it for 12 months even in stored at 2-8C and can use it several times. 2) The assay range has changed from 2.15 - 275 ng/mL to 1.09 - 70 ng/mL (sensitivity is 0.25 ng/mL). Additionally, we recommend the customers to use IBLysis-I (# 19022) instead of TNE buffer for sample treatment. IBLysis-I was developed by improving TNE buffer solution for solubilization of cultured cells and tissue. It is ready to use for pretreatment of samples. Cyclooxygenase (COX) is a membrane bound enzyme responsible for the oxidation of arachidonic acid to Prostaglandin G2 (PGG2) and the subsequent reduction of PGG2 to PHG2. These reactions are the first steps in the formation of a variety of prostanoids. COX has been shown to be expressed in at least two different isoforms, a constitutively expressed form, COX-1, and an inducible form, COX-2. COX-1 is thought to regulate a number of housekeeping functions, such as vascular hemostasis, renal blood flow, and maintenance of glomerular function. Inflammation mediators such as growth factors, cytokines and endotoxin induce COX-2 expression in a number of cellular systems.


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