Catalogue Number: 32-1048-ABO
Manufacturer: | Abeomics |
Type: | Other Native Protein |
Alias: | CLI||AAG4||KUB1||SGP2||SGP-2||SP-40||TRPM2||MGC24903||Clusterin||Apolipoprotein J||Apo-J. |
Host Cell: | Plasma |
Shipping Condition: | Dry Ice |
Unit(s): | 10 ug |
Description: Source : Plasma. Clusterin also named Apolipoprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified.The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide & subsequently between residues 227/228 to generate the a & b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges & are flanked by two predicted coiled-coil a-helices & three predicted amphipathic a-helices.Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues & can be found in plasma, milk, urine, cerebrospinal fluid & semen.It is able to bind & form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin & others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodeling, lipid transport, hormone transport &/or scavenging, matrix metalloproteinase inhibition.A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced insults caused by degraded & misfolded protein precipitates.Clusterin is up- or down regulated on the mRNA or protein level in many pathological & clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity & others.
It is recommended to add deionized H2O to prepare a working stock solution of 0.5mg/ml & let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.