Catalogue Number: 32-1076-ABO
Manufacturer: | Abeomics |
Type: | Other Recombinant Protein |
Alias: | BAFF||BLYS||CD257||TALL1||THANK||ZTNF4||TALL-1||TNFSF20||TNFSF13B||B-cell Activating Factor. |
Shipping Condition: | RT |
Unit(s): | 5 ug |
Description: Source : Nicotiana benthamiana plant BAFF human Recombinant produced in Nicotiana benthamiana plant is a single glycosilated polypeptide chain containing 151 amino acids fragment (134-285).BAFF (C830H1277N223O242S5) is fused to a 10-His-tag at the N-terminal having the total molecular mass of 18-20kDa & purified by st&ard chromatographic techniques. BAFF binds to tnfrsf13b/taci & tnfrsf17/bcma. Tnfsf13/april binds to the same 2 receptors, together, they form a 2 lig&s -2 receptors pathway involved in the stimulation of b- & t-cell function & the regulation of humoral immunity.A third b-cell specific baff-receptor (baffr/br3) promotes the survival of mature b-cells & the b-cell response.B Lymphocyte Stimulator functions as a potent B-cell growth factor in costimulation assays. Administration of BAFF Human recombinant to mice disrupts splenic B-cell & T-cell zones & results in elevated levels of serum immunoglobulin.
It is recommended to reconstitute the lyophilized BAFF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The activity is determined by dose-dependent stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method. *activity results may vary with PBMC donors. ED50 ? 50ng/ml