LIF Yeast Recombinant Protein
Catalogue Number: 32-1583-ABO
| Manufacturer: | Abeomics |
| Type: | Other Recombinant Protein |
| Alias: | CDF||HILDA||D-FACTOR||Differentiation- stimulating factor||Melanoma-derived LPL inhibitor||MLPLI||Emfilermin||Leukemia inhibitory factor||LIF||DIA. |
| Shipping Condition: | RT |
| Unit(s): | 25 ug |
Description
Description: Source : Pichia pastoris. LIF Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 180 amino acids & having a molecular mass of 58.5 kDa. The LIF is purified by proprietary chromatographic techniques. Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human & mouse LIF exhibit a 78% identity in its amino acid sequence.
Additional Text
Application Notes
It is recommended to reconstitute the lyophilized LIF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The biological activity of recombinant human LIF was measured by the ability to induce differentiation of murine M1 myeloid leukemic cells. The minimal detectable concentration of human LIF in this assay is 1 x 108 units/mg.
32-1583 Datasheet
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