SCF Recombinant Protein

Catalogue Number: 32-1743-ABO

Manufacturer:Abeomics
Type:Ligand
Alias:Kit ligand Precursor,C-kit ligand,SCF,Mast cell growth factor,MGF,SF,KL-1,Kitl,DKFZp686F2250.
Host Cell:E. Coli
Shipping Condition:Blue Ice
Unit(s): 10 ug

Description

Description: Source : Escherichia Coli.Stem Cell Factor Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is purified by proprietary chromatographic techniques.Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as 'steel factor' or 'c-kit ligand'. SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

Additional Text

Application Dilution

It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000 IU/mg.

Concentration

Lyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3.

Amino Acid Sequnce

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys.