Avidin Native Protein
Catalogue Number: 32-3274-ABO
| Manufacturer: | Abeomics |
| Type: | Other Native Protein |
| Alias: | Avidin||AVD||AVID. |
| Host Cell: | Hens Egg White |
| Shipping Condition: | RT |
| Unit(s): | 25 mg |
Description
Description: Source : Hen's egg white. Avidin is a tetrameric protein of 4 identical subunits (homotetramer) each of which can bind to biotin with a high degree of affinity and specificity. Avidin molecular weight in its tetrameric form is estimated to be between 66-69 kDa. Avidin is produced in the oviducts of birds, reptiles and amphibians and is subsequently deposited in the whites of their eggs. In the chicken egg white, avidin makes up roughly 0.05% of total protein (approximately 1.8 mg per egg). 10% of Avidin's molecular weight is ascribed to carbohydrate content which is composed of four to five mannose and three N-acetylglucosamine residues. Avidin has at least three distinctive oligosaccharide structural type which are similar in structure and composition. The dissociation constant (KD) of avidin is approximately 10-15M, making it one of the strongest known non-covalent bonds.
Additional Text
Application Notes
It is recommended to reconstitute the lyophilized Avidin in sterile 18MΩ-cm H2O not less than 100µg/ml or more than 10mg/ml solutions. 16 units/mg protein, 1 unit binds 1µg biotin.
32-3274 Datasheet
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