Rabbit polyclonal antibody to SARS-CoV2 S-protein (ACE2 binding domain)
Catalogue Number: 34-1136-ABO
34-1136 Image
34-1136 Image
34-1136 Image
Fig 1 :Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector (5) including DNA encoding the SARS-CoV2 S-protein ACE2 binding domain, amino acids 308-541 in the S-protein sequence in Wuhan-Hu-1, complete genome. This region was used as the immunogen for development of rabbit antibody RPCA-SARS-CoV2-bd. The antibody was used at a dilution 1:3,000, shown in red. Cells costained with mouse mAb to Beta -tubulin, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. The SARS-CoV2-bd antibody cleanly reveals expression of the SARS-CoV2-bd protein only in transfected cells. The Beta -tubulin antibody produces strong staining of microtubules in the cytoplasm in both transfected and non-transfected cells.
34-1136 Image
Fig 2 :Western blot analysis of HEK293 cell lysates using rabbit pAb to SARS-CoV2-bd protein, SARS-CoV-bd, dilution 1:2,000 in green: [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing the SARS-Cov-bd cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing the SARS-CoV-bd cDNA. The band at 25kDa in lane 2 demonstrates expression of SARS-CoV-bd protein, and the band at about 50kDa in lane 3 corresponds to GFP-SARS-CoV-bd fusion protein. The same blot was simultaneously probed with mouse mAb to Beta -tubulin, dilution 1:5,000, in red, which reveals a single band at 50kDa in both transfected and non-transfected cells.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Application: | ICC, IF, WB |
Description
Description: This antibody was raised against a recombinant construct of the SARS-CoV-2 spike or S-protein which includes the entire region which interacts with ACE2. The specific binding to ACE2 is essential for viral internalization and infection. We designed this construct based on amino acids 308-541 in the S-protein sequence in Isolate Wuhan-Hu-1, complete genome. This is a defined globular domain recently shown to include all of the amino acids necessary for ACE2 binding. The construct was expressed in and purified from E. coli and includes an N-terminal His-tag and other vector derived sequence shown underlined below. Amino acids which interact directly with the ACE2 protein are printed in bold.
Additional Text
Antibody Clonality
Polyclonal
Application Notes
WB: 1:1,000-1:2,000. ICC/IF: 1:3,000-5,000
34-1136 Datasheet
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