Polyclonal Antibody to eIF4E (Phospho-Ser209)
Catalogue Number: 35-1218-ABO
35-1218 Image
35-1218 Image
35-1218 Image
35-1218 Image
Figure 1: Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody 35-1218 .
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Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E(Phospho-Ser209) Antibody 35-1218 (left) or the same antibody preincubated with blocking peptide(right).
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Figure 3: Immunofluorescence staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) Antibody 35-1218 .
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | mRNA cap-binding protein|| eIF-4F 25 kDa subunit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E. |
| Application: | IF, WB, IHC |
Description
Description: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Li BD, et al. (1998) Ann Surg; 227(5): 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520 De Gregorio E, et al. (2001) RNA; 7(1): 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461
Additional Text
Gene ID
1977
Gene Name
EIF4E
Uniprot ID
P06730
Antibody Clonality
Polyclonal
Application Notes
Predicted MW: 25kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
35-1218 Datasheet
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