Polyclonal Antibody to Histone H2A.X (Phospho-Ser139)
Catalogue Number: 35-1247-ABO
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35-1247 Image
35-1247 Image
35-1247 Image
Figure 1: Western blot analysis of extracts from HT29 cells untreated(lane 1) or treated with UV(lane 2) using Histone H2A.X(Phospho-Ser139) Antibody 35-1247 .
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Figure 2: Immunofluorescence staining of methanol-fixed Hela cells using Histone H2A.X(Phospho-Ser139) Antibody 35-1247 .
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Figure 3: Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using Histone H2A.X (Phospho-Ser139) Antibody 35-1247 .
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | H2A.X|| H2AFX|| H2a/x|| HIST5-2AX |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X. |
| Application: | IF, WB |
Description
Description: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, imiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to ow doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330. Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6.
Additional Text
Gene ID
3014
Uniprot ID
P16104
Gene Name
H2ax
Antibody Clonality
Polyclonal
Application Notes
Predicted MW: 15kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
35-1247 Datasheet
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