Anti-LC1 Liver Cytosol(lgG) EIA, 96 wells, "RUO"
Catalogue Number: 35-LC1HU-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Human ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 3 - 300 U/mL |
| Sensitivity: | 1 U/mL |
| Sample type: | Serum |
| Sample size: | |
| Application: | IA, EIA |
Description
Description: The Anti-LC1 ELISA is intended for the quantitative and qualitative determination of anti-liver cytosol type 1 autoantibodies in human serum. - Ready-to-use controls and calibrators - Results in only 90 minutes Research Use Only. Not for Use in Diagnostic Procedures. Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Anti LC-1 antibodies, if present in the sample, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The rate of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.
Additional Text
Storage Note
store at 2-8C
35-LC1HU-E01 Protocol
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