BRAF, NT (B-Raf, Serine/threonine-protein Kinase B-raf)

Catalogue Number: 351773-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 100ug/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Rabbit
Clone:	D9T6S
Isotype:	IgG
Immunogen:	Recombinant protein corresponding to human B-Raf at N-terminal.
Application:	IP, WB

Overview
Datasheets

Description

Description: A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events (11).

Additional Text

Gene Name

BRAF

Uniprot ID

P15056

Gene ID

673

Specificity

Recognizes human B-Raf. Species Crossreactivity: monkey, mouse, rat

Purification

Purified

Antibody Clonality

Monoclonal

351773 Datasheet

Price

£592.00

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