Screen Quest™ Fluorimetric ELISA cAMP Assay Kit
Catalogue Number: 36374-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Small Molecules ELISA Kits |
| Shipping Condition: | Blue Ice |
| Unit(s): | 10 plates |
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Description
Description: Screen Quest™ Fluorescence ELISA cAMP Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. The assay is based on the competition for a fixed number of antibody binding sites between HRP-labeled cAMP and non-labeled cAMP. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test sample competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. Our Screen Quest™ Fluorometric cAMP Assay Kit provides a sensitive method for detecting adenylate cyclase activity. Compared to other commercial ELISA cAMP assay kits, this cAMP assay kit only requires a single wash step to remove unbound material prior to the development step. It also eliminates the tedious acetylation step. The kit uses Amplite™ Red as a fluorogenic HRP substrate to quantify the HRP activity. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate.
Additional Text
Storage Note
Multiple;|6 months
36374 Protocol
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