Catalogue Number: 40589-V49H3-B-SIB
|Molecular Weight:||The recombinant SARS-CoV-2 Spike S1+S2 consists of 1243 amino acids and predicts a molecular mass of 138.48 kDa.|
|Shipping Condition:||Blue Ice|
Description: A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations A67V, HV69-70 del, T95I, G142D, VYY143-145 del, N211 del, L212I, ins 214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, F817P, N856K, A892P, A899P, A942P, Q954H, N969K, L981F, K986P, V987P and furin cleavage site mutants) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.529) which emerged in the South Africa. The recombinant SARS-CoV-2 Spike S1+S2 consists of 1243 amino acids and predicts a molecular mass of 138.48 kDa.
AviTag™, HIS Tag
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.