Ceruloplasmin ELISA
Catalogue Number: 41-CERHU-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Human ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 3.1 ng/ml - 200 ng/ml |
| Sensitivity: | 3.1 ng/ml |
| Sample type: | Plasma, Serum |
| Sample size: | |
| Application: | ELISA, IA |
Description
Description: In this assay the ceruloplasmin (Cp) present in samples reacts with the anti-Cp antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Cp, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added, and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Cp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Cp in the test sample. The quantity of Cp in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
Additional Text
Storage Note
store at 2-8C
41-CERHU-E01 Protocol
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