Rabbit C-Reactive Protein EIA, (96 wells),"RUO"
Catalogue Number: 41-CRPRB-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Rabbit ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 6.25 - 200 ng/mL |
| Sensitivity: | 5 ng/mL |
| Sample type: | Plasma, Serum |
| Sample size: | 2 µL |
| Application: | IA, EIA |
Description
Description: In this assay, the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, bound enzyme is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
Rabbit C-Reactive Protein ELISA (Rabbit CRP ELISA)
Storage Note
store at 2-8C, ref. serum -20C
41-CRPRB-E01 Protocol
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