IgA (Chicken) ELISA, 96w, RUO
Catalogue Number: 41-IGACH-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Chicken ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 12.5-400 ng/mL |
| Sensitivity: | 12.5 ng/mL |
| Sample type: | Plasma, Serum |
| Sample size: | 100 µL |
| Application: | ELISA, IA |
Description
Description: In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
IgA (Chicken) ELISA
Storage Note
store at 2-8C, ref. serum -20C
41-IGACH-E01 Protocol
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