KIM-1 Mouse ELISA, (96 wells), "RUO"
Catalogue Number: 41-KIMMS-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Mouse ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 0.078 - 5 ng/mL |
| Sensitivity: | 0.078 ng/mL |
| Sample type: | Serum, Urine |
| Sample size: | 100 µL |
| Application: | ELISA, IA |
Description
Description: KIM-1 is a trans-membrane structural glycoprotein in renal epithelial cells. These cells will shed KIM-1 antigen during regeneration into the urine which makes urinary KIM-1 a useful biomarker for kidney injury. In this assay, the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-KIM-1 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound KIM-1. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Additional Text
Short Description
The Mouse KIM-1 ELISA is a highly sensitive two-site enzyme linked immunoassay for measuring KIM-1 in biological samples of mice.
Storage Note
store at 2-8C
41-KIMMS-E01 Protocol
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