KIM-1 (Rat) ELISA, 96w, RUO
Catalogue Number: 41-KIMRT-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Rat ELISA Kit |
| Shipping Condition: | RT |
| Unit(s): | 96 Wells |
| Range: | 0.156-10 ng/mL |
| Sensitivity: | 0.156 ng/mL |
| Sample type: | Other Biological Sample, Serum |
| Sample size: | 50 µL |
| Application: | ELISA, IA |
Description
Description: In this assay, the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-KIM-1 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound KIM-1. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
The Rat KIM-1 ELISA is a highly sensitive two-site enzyme linked immunoassay for measuring KIM-1 in biological samples of rats.
41-KIMRT-E01 Protocol
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