Rabbit Polyclonal Antibody to GFP
Catalogue Number: 45-1042-ABO
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Figure-1 : Western blot analysis of GFP Antibody at 1 µg/ml on GFP fusion protein (200, 100, 50 & 10 ng), IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
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Figure-4 : Western blot analysis of GFP Antibody on immunoprecipitates from lysate expressing GFP, 1: Input control material for lysate expressing GFP protein, 2: Negative control with isotype control, 3: Immunprecipitation with GFP Antibody.
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Figure-2 : Figure-2: Western Blot Analysis of GFP Antibody at 1 µg/ml on GFP uv fusion protein (100, 40, 20 & 10 ng), IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
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Figure-3 :Western Blot Analysis of GFP Antibody at 1 µg/ml on YFP fusion protein, IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
| Manufacturer: | Abeomics |
| Type: | Tag antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Purified recombinant full-length GFP protein |
| Application: | ELISA, WB |
Description
Description: Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants. Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Application Notes
ELISA: 0.05-0.2 µg/ml.Western blot: 1-2 µg/ml
45-1042 Datasheet
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