iFluor® 514 Styramide *Superior Replacement for Opal 540*

Catalogue Number: 45022-AAT

Manufacturer:AAT Bioquest
Shelf Life:12 months
Type:Fluorescent Reagents
Shipping Condition:RT
Unit(s): 100 slides

Description

Description: Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher fluorescence intensity, increased photostability, and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for the covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust, and more sensitive than traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates can label the target at higher efficiency and thus generate a significantly higher fluorescence signal. Styramide™ conjugates also allow substantially less consumption of primary antibodies compared to the standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 514 Styramide is optimized to be a superior replacement for Opal Polaris 540 or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Additional Text

Storage Note

Freeze (< -15 °C); Minimize light exposure;|12 months