iFluor® 670 Styramide *Replacement for Opal 690*

Catalogue Number: 45047-AAT

Manufacturer:AAT Bioquest
Shelf Life:12 months
Type:Fluorophores
Shipping Condition:RT
Unit(s): 100 slides

Description

Description: The Power Styramide™ Signal Amplification (PSA™) system is a highly sensitive method for detecting low-abundant targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH). When combined with bright and photostable iFluor® dyes, Styramide™ conjugates offer sharper and more precise results, exceeding the sensitivity of standard ICC/IHC/ISH methods by over 100 times, all while reducing the consumption of primary antibodies. Like tyramide signal amplification (TSA), PSA™ also uses the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. However, Styramide™ radicals are more reactive than tyramide radicals, making the labeling of the target protein or nucleic acid sequence faster and more robust. This provides fluorescence signals that are 10-50 times greater than those generated by tyramide (TSA) reagents. iFluor® 670 Styramide™ uses the bright red-fluorescent dye iFluor® 670 (Ex/Em = 671/682 nm) to label targets in situ. The high fluorescence quantum yield and photostability of iFluor® 670 styramide outperforms Opal 690 tyramide and other spectrally similar reagents, making it a superior alternative for imaging applications.

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Storage Note

Freeze (< -15 °C); Minimize light exposure;|12 months