Catalogue Number: 45047-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorophores |
| Shipping Condition: | RT |
| Unit(s): | 100 slides |
Description: The Power Styramide™ Signal Amplification (PSA™) system is a highly sensitive method for detecting low-abundant targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH). When combined with bright and photostable iFluor® dyes, Styramide™ conjugates offer sharper and more precise results, exceeding the sensitivity of standard ICC/IHC/ISH methods by over 100 times, all while reducing the consumption of primary antibodies. Like tyramide signal amplification (TSA), PSA™ also uses the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. However, Styramide™ radicals are more reactive than tyramide radicals, making the labeling of the target protein or nucleic acid sequence faster and more robust. This provides fluorescence signals that are 10-50 times greater than those generated by tyramide (TSA) reagents. iFluor® 670 Styramide™ uses the bright red-fluorescent dye iFluor® 670 (Ex/Em = 671/682 nm) to label targets in situ. The high fluorescence quantum yield and photostability of iFluor® 670 styramide outperforms Opal 690 tyramide and other spectrally similar reagents, making it a superior alternative for imaging applications.
Freeze (< -15 °C); Minimize light exposure;|12 months