mFluor™ Blue 660 Styramide
Catalogue Number: 45074-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorophores |
| Shipping Condition: | RT |
| Unit(s): | 100 slides |
Description
Description: The Power Styramide™ Signal Amplification (PSA™) system is a highly sensitive method for detecting low-abundant targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH). By utilizing bright and photostable mFluor™ dyes, Styramide™ conjugates deliver results of unparalleled sharpness and precision, surpassing the sensitivity of standard ICC/IHC/ISH methods by over 100 times, all the while reducing the consumption of primary antibodies. Like tyramide signal amplification (TSA), PSA™ leverages the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The enhanced reactivity of Styramide™ radicals over tyramide ensures faster, more robust labeling of the target, leading to fluorescence signals that are 10-50 times greater than those generated by tyramide (TSA) reagents. The mFluor™ Blue 660 Styramide uses the bright red fluorescent dye mFluor™ Blue 660 (Ex/Em = 481/663 nm) to label targets in situ. mFluor™ Blue 660 is well-excited by the 488 nm laser line and exhibits minimal crosstalk in complex multicolor analysis with blue and green fluorescent probes.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
45074 Protocol
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