iFluor® 450 Tyramide *Superior Replacement for Opal 480*
Catalogue Number: 45097-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 200 slides |
Description
Description: For many immunohistochemical (IHC) applications, traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit their sensitivity and utility. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label results in the ability to detect low-abundance targets with ultrasensitive precision and reduces the amount of antibodies and hybridization probes needed. In IHC applications, this method can also enhance sensitivity in cases where the primary antibody dilution needs to be increased to reduce nonspecific background signals or overcome weak immunolabeling due to suboptimal fixation procedures or low levels of target expression. The iFluor® 450 tyramide contains the bright iFluor® 450 that can be readily detected with the standard FITC filter set. The iFluor® dyes offer brighter fluorescence intensities, increased photostability, and enhanced water solubility, leading to fluorescence signals with significantly higher precision and sensitivity. iFluor® 450 is an excellent replacement for Opal 480 or other spectrally similar fluorescent tyramide conjugates.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
45097 MSDS
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