FuGENE® 4K Transfection Reagent
Catalogue Number: 4K-1000-FUG
4K-1000 Image
4K-1000 FuGENE® 4K reagent outperforms Lipofectamine® 3000
4K-1000 Image
4K-1000 FuGENE® 4K reagent outperforms Lipofectamine® 3000
A549, C2C12, HCT116, CHO-K1, HeLa, NIH3T3, LNCaP, PANC1 & SKOV3 cell lines were seeded in 96-well plates according to user guides and subsequently transfected with FuGENE® 4K or Lipofectamine® 3000 (registered trademark of Thermo Fisher Scientific) and a GFP expressing plasmid at the following transfection reagent to DNA ratios (3:1, 4:1 & 5:1). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % GFP expressing cells and % total cell viability (via propidium iodide dye). The graphs above show the superior performance of FuGENE® 4K vs. Lipofectamine® 3000.
| Manufacturer: | Fugene |
| Shelf Life: | 2 years |
| Physical state: | Solution |
| Type: | Transfection Reagents and Kit |
| Shipping Condition: | RT |
| Storage Condition: | 4°C |
| Unit(s): | 1 ml |
Description
Description: FuGENE® 4K is the most advance, best-in-class, multicomponent transfection reagent for high efficiency, and low toxicity delivery of DNA into common and difficult-to-transfect eukaryotic cells. FuGENE® 4K enables researchers to obtain superior DNA transfection efficiencies across applications such as gene expression, protein production, and viral production. The simple, easy-to-use protocol with minimal optimization allows users to free up valuable lab time.Number of transfection experimentsIn a typical experiment, 1ml of FuGENE® 4K Transfection reagent can be used to perform up to 3,300 transfections in one well of
4K-1000 Technical Manual
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