Buccutite™ Rapid APC-Cy7 Tandem Antibody Labeling Kit *Production Scale Optimized for Labeling 1 mg Antibody Per Reaction*
Catalogue Number: 5415-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Conjugation Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 2 labelings |
Description
Description: Buccutite™ Rapid APC-Cy7 Tandem Antibody Labeling Kits, designed for large-scale production, provide a streamlined approach for labeling antibodies with PE, APC, PerCP, and iFluor® tandem dyes. Compared to conventional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite™ conjugation is simple and more robust. Using a two-step mixing protocol, researchers can directly conjugate APC-Cy7 to any antibody or protein in less than 2 hours. Each Buccutite™ kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column for maximum conjugate yield. Each Buccutite™ FOL-Activated APC-Cy7 vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Allophycocyanin-cyanine 7 (APC-Cy7) is an intensely bright, NIR fluorescent tandem fluorophore with an excitation and emission maxima of ~651 nm and ~779 nm, respectively. Given its intense brightness, APC-Cy7 is recommended for pairing with low-abundance targets to minimize spillover and compensation. APC-Cy7 conjugates are well-suited for flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.
Additional Text
Storage Note
Multiple;|6 months
5415 MSDS
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