Strep-Tactin® APC
Catalogue Number: 6-5010-001-IBA
| Manufacturer: | IBA GmbH |
| Shelf Life: | 12 Months |
| Type: | Avidin/Streptavidin Biotin Products |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 50 ul |
Description
Description: Strep-Tactin® conjugated to either R-Phycoerythrin (PE) or Allophycocyanin (APC) is suitable for staining or isolation of antigen-specific cytotoxic T cells (MHC I Streptamer®) or surface marker-specific cells (Fab-TACS®/Nano-TACS®) based on our Strep-tag® technology. To target specific cells, MHC I complexes, low affinity Fab fragments or nanobodies fused to a Twin-Strep-tag® (MHC I-Streps, Fab-Streps or Nano-Streps) are required in addition to fluorescent Strep-Tactin®. Strep-Tactin® multimerizes MHC I-Streps or Fab-/Nano-Streps, ensuring stable binding to cells. The resulting fluorescent complexes can be used to stain cells that are specific for the respective peptide-loaded MHC I molecule or for a selected surface marker. The addition of biotin reverses the multimerization and causes the dissociation of MHC I-Streps or Fab-/Nano-Streps from the cell surface. The complete removal of all labeling reagents preserves the cells' full biological activity.
Additional Text
Short Description
Fluorescently conjugated Strep-Tactin® for cell staining; for 0.5-1x10^8 cells.
SUPPORT
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PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations