6-HEX phosphoramidite [5'-Hexachlorofluorescein phosphoramidite]

Catalogue Number: 6026-AAT

Manufacturer:AAT Bioquest
Shelf Life:6 months
Type:Fluorescent Reagents
Shipping Condition:RT
Unit(s): 100 umoles

Description

Description: There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5’-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5’-hexachlorofluorescein-CE phosphoramidite (HEX) and 5’-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5’-end. Labeling the 3’-end of an oligo with fluorescein can be achieved using 3’-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.

Additional Text

Storage Note

Freeze (< -15 °C); Minimize light exposure;|6 months