Biotin-SP-conjugated AffiniPure VHH Fragment Alpaca Anti-Rabbit IgG (H+L) (min X Bov, Hu, Ms Sr Prot)
Catalogue Number: 611-064-215-JIR
| Manufacturer: | Jackson ImmunoResearch |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Freeze-dried solid |
| Type: | VHH Nanobodies |
| Shipping Condition: | RT |
| Unit(s): | 0.1 ml |
| Host name: | Alpaca |
| Clone: | |
| Isotype: | |
| Immunogen: | IgG (H+L) |
| Application: | ELISA, FC, ICC, WB, IHC |
Description
Description: Nano Secondaries are polyclonal VHH Fragment antibodies (Nanobodies) produced in alpacas. 10x smaller than conventional whole IgG antibodies, the
Additional Text
Antibody Clonality
Polyclonal
Application Notes
1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Storage Note
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
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