Catalogue Number: 615-064-214-JIR
Manufacturer: | Jackson ImmunoResearch |
Preservative: | 0.05% Sodium azide |
Physical state: | Freeze-dried solid |
Type: | VHH Nanobodies |
Shipping Condition: | RT |
Unit(s): | 0.1 ml |
Host name: | Alpaca |
Clone: | |
Isotype: | |
Immunogen: | IgG (H+L) |
Application: | ELISA, FC, ICC, WB, IHC |
Description: Nano Secondaries are polyclonal VHH Fragment antibodies (Nanobodies) produced in alpacas. 10x smaller than conventional whole IgG antibodies, the
Polyclonal
1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.