Alkaline Phosphatase-conjugated AffiniPure® F(ab')2 Fragment Donkey Anti-Guinea Pig IgG (H+L) (min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)

Catalogue Number: 706-056-148-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	12 months
Preservative:	0.05% Sodium azide
Physical state:	Freeze-dried solid
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	0.3 ml
Host name:	Donkey
Clone:
Isotype:
Immunogen:	IgG (H+L)

Overview
Datasheets

Description

Description: F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, syrian hamster, horse, human, mouse, rabbit, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

Additional Text

Purification

AffiniPure

Antibody Clonality

Polyclonal

Short Description

Donkey Anti-Guinea Pig IgG (H+L) secondary antibody, conjugated to Alkaline Phosphatase. This is an affinity purified F(ab′)2 Fragment polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine, Chicken, Goat, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep serum proteins.

Application Notes

ELISA:- 1:5,000-1:50,000 Western Blot:- 1:5,000-1:50,000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.