5-OG488 succinimidyl ester [equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer ]
Catalogue Number: 710-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorophores |
| Shipping Condition: | RT |
| Unit(s): | 5 mg |
Description
Description: 5-OG488 succinimidyl ester is the same molecule to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer (Invitrogen #O6147, Oregon Green® is the Trademark of Invitrogen). This fluorinated analog of fluorescein has higher photostability and a lower pKa ~ 4.7 while 5-FAM has pKa ~6.4. The decreased pKa of 5-OG488 makes its fluorescence essentially pH insensitive in the physiological pH range. This amine-reactive 5-OG488 succinimidyl ester can be used to make bright green fluorescent bioconjugates with excitation/emission maxima ~496/524 nm. It is an excellent replacement to 5-FITC that has severe pH dependence and low photostability.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
710 Protocol
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