6 nm Colloidal Gold-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (EM Grade)

Catalogue Number: 715-195-150-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	6 months
Preservative:	0.05% Sodium azide
Physical state:	Liquid
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	0.3 ml
Host name:	Donkey
Clone:
Isotype:
Immunogen:	IgG (H+L)
Application:	ICC, IHC

Overview
Datasheets

Description

Description: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

Additional Text

Purification

AffiniPure

Antibody Clonality

Polyclonal

Short Description

Donkey Anti-Mouse IgG (H+L) secondary antibody, conjugated to 6 nm Colloidal Gold . This is an affinity purified whole IgG polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine, Chicken, Goat, Guniea Pig, Syrian Hamster, Horse, Human, Rabbit and Sheep serum proteins.

Application Notes

Histo-/Cyto-Chemistry:- 1:20-1:40 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Storage Note

Storage: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution on day of use. Expiration date: six months from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Product Specification Sheets