cAMP Complete ELISA, 5x96 Wells, "RUO" Ship & Store some components at -20°C.
Catalogue Number: 74-CAMHU-E05-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Human ELISA Kit |
| Shipping Condition: | Dry Ice |
| Unit(s): | 5 x 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | See Package Insert |
| Sensitivity: | See Package Insert |
| Sample type: | Cell Culture, Saliva, Serum |
| Sample size: | 25 µL |
| Application: | ELISA, IA |
Description
Description: This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cAMP diluted in buffer or intracellular cAMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
The cAMP Complete ELISA is a colorimetric competitive immunoassay for the quantitative determination of extracellular cAMP in cell lysates, culture supernatants, serum, saliva, and tissue. This format includes five 96 well plates. Reagents included Sensitivity is increased 10-fold by acetylation Absorbance is read at 405 nm Large signal-to-background ratio offers superior sensitivity Easily assess adenylyl cyclase activation by GPCRs
Storage Note
Select components are stored at -20°C
74-CAMHU-E05 Protocol
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