Ultrasensitive Corticosterone ELISA, 96 wells, "RUO"
Catalogue Number: 74-CORMSU-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Rodent ELISA kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 30 - 20,000 pg/mL |
| Sensitivity: | 27.0 pg/mL |
| Sample type: | Cell Culture, Other Biological Fluid, Plasma, Serum |
| Sample size: | |
| Application: | ELISA, IA |
Description
Description: The Corticosterone Enzyme-Linked Immunosorbent Assay (ELISA) kit is a competitive immunoassay for the quantitative determination of Corticosterone in biological fluids. The kit uses a polyclonal antibody to Corticosterone to bind, in a competitive manner, Corticosterone in the standard or sample or an alkaline phosphatase molecule which has Corticosterone covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Corticosterone in either standards or samples. The measured optical density is used to calculate the concentration of Corticosterone.
Additional Text
Storage Note
store at 2-8C
74-CORMSU-E01 Protocol
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