Catalogue Number: 74-DPPHU-E01-ALP
Manufacturer: | Alpco Diagnostics |
Type: | Immunodetection Kits and Reagents |
Shipping Condition: | RT |
Storage Condition: | -70°C |
Unit(s): | 96 tests |
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Application: | IA |
Description: The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, providing enough material to perform at least 96 assays. DPPIV (DPP4, CD26) is a member of the class of proteases known as prolyl peptidases, which cleave proteins after proline residues and is thought to play roles in diabetes, cancer, and autoimmune diseases, making it a target for drug discovery. The kit contains both a chromogenic substrate (H-Gly-Pro-pNA; Km=114 uM) and a fluorogenic substrate (H-Gly-Pro-AMC; Km=50 uM). Cleavage of the p-nitroaniline (pNA) from the colorimetric substrate increases absorbance at 405 nm. The fluorimetric assay is based on the cleavage of 7-amino-4-methylcoumarin (AMC) moiety from the C-terminus of the peptide substrate, which increases its fluorescence intensity at 460 nm. The kit is useful to screen inhibitors of DPPIV, a potential therapeutic target. A DPPIV inhibitor, P32/98 (KI=130 nM12), is included for use as a control. Other DPP enzymes are available for specificity profiling. The DPPIV Drug Discovery Kit is for Research Use Only. For more information on the DPPIV Drug Discovery Kit, call 800-592-5726.
Research Use Only. Not for Use in Diagnostic Procedures.
DPPIV Drug Discovery Kit
Store at -80°C