Vimentin Anti-Human Monoclonal [Clone VM1170]

Catalogue Number: 7431-MSM3-P1-EQB

Manufacturer:Enquire Bioreagents
Shelf Life:2 years
Preservative:BSA and Azide
Physical state:Liquid
Type:Monoclonal Primary Antibody
Alias:VIM; anti-VIM antibody
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Mouse
Clone: VM1170
Isotype: IgG1
Immunogen: Recombinant full-length human vimentin protein

Additional Text

Hu-Chromosome Location

10p12.33

Gene Symbol

VIM

Concentration

200ug/ml

Additional application information

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Molecular Weight

57-60kDa

Specificity

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.