ZAP70 Anti-Human Monoclonal [Clone 2F3.2]

Catalogue Number: 7535-MSM1-P1-EQB

Manufacturer:Enquire Bioreagents
Shelf Life:2 years
Preservative:BSA and Azide
Physical state:Liquid
Type:Monoclonal Primary Antibody
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Mouse
Clone: 2F3.2
Isotype: IgG2a, kappa
Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70

Additional Text

Molecular Weight

70kDa

Hu-Chromosome Location

2q12

Gene Symbol

ZAP70

Concentration

200ug/ml

Additional application information

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Specificity

ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.