C-Peptide (Mouse) ELISA, 96 wells, "RUO"

Catalogue Number: 80-CPTMS-E01-ALP

Manufacturer:	Alpco Diagnostics
Type:	Mouse ELISA Kit
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	96 Wells
Host name:
Clone:
Isotype:
Immunogen:
Range:	60 - 3000 pM
Sensitivity:	7.6 pM
Sample type:	Serum
Sample size:	10 µL
Application:	ELISA, IA

Overview
Datasheets

Description

Description: The ALPCO Mouse C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the conjugate. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. TMB Substrate is added, and the microplate is incubated a second time at room temperature on a microplate shaker at 700-900 rpm. Once the second incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.

Additional Text

Regulatory Statuses

Research Use Only. Not for Use in Diagnostic Procedures.

Short Description

The Mouse C-peptide ELISA quantifies the C-peptide protein products of mouse I and mouse II proinsulin genes. The assay utilizes a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips. A single Mouse C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. Only 10 uL of sample is sufficient for the assay Superior analytical sensitivity of 7.6 pmol/L Broad d

Storage Note

store at 2-8C

80-CPTMS-E01 Protocol

Price

£607.00

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