C-Peptide (Rat) ELISA, 96 wells, "RUO"
Catalogue Number: 80-CPTRT-E01-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Rat ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 50 - 4500 pM |
| Sensitivity: | 10.8 pM |
| Sample type: | Serum |
| Sample size: | 10 µL |
| Application: | ELISA, IA |
Description
Description: The ALPCO Rat C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the conjugate. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. TMB Substrate is added, and the microplate is incubated a second time at room temperature on a microplate shaker at 700-900 rpm. Once the second incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
The Rat C-peptide ELISA quantifies the C-peptide protein products of rat I and rat II proinsulin genes. Only 10 uL of sample is sufficient for the assay Superior sensitivity of 10.8 pM Dynamic range of 50-4,500 pM
Storage Note
store at 2-8C
80-CPTRT-E01 Protocol
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