Mouse anti Human CD8 FITC - CD45RA PE

Catalogue Number: 845RA-NMB

Manufacturer:	Nordic MuBio
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	CD8 FITC - CD45RA PE
Shipping Condition:	RT
Unit(s):	100 tests
Host name:	Mouse
Clone:	17D8/BHKG-2
Isotype:	IgG1
Immunogen:	CD45=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Application:	FC

Overview

Description

Description: CD8 is useful for the Identification of human T cells suppressor/cytotoxic expressing the 32 kDa M.W. surface antigen. While CD45RA recognizes MW. 220-kdalton of the leucocyte antigen (LCA). CD45RA antigen is a member of the CD45 antigen family that also includes CD45, CD45RO, and CD45RB antigens. CD45RA is expressed on approximately 75% of CD8 lymphocytes and 50% of CD4 lymphocytes, all B cells and NK Cells. CD45RA antigen is expressed on naive T lymphocytes, antigen density decreases upon in vitro activation. A decrease antigen density has been demonstrated for CD4+CD45RA+subset with active multiple sclerosis.

Additional Text

Gene ID

925

Gene Name

CD8A

Uniprot ID

P01732

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

Add 10 µl of MAB / 100 µl of 106 PBMC in PBS. Mix Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Add 10 µlof MAB / 100 µl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse Whole Blood and analyze or fix with 0.5% v/v paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Storage Note

Product should be stored at 4-8C. DO NOT FREEZE

Price

£408.00

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