Human VEGF (Vascular Endothelial Cell Growth Factor) Pre-Coated ELISA Kit

Catalogue Number: 90-2138-ABO

Manufacturer:	Abeomics
Type:	Human ELISA Kit
Shipping Condition:	Blue Ice
Unit(s):	1 x 96 Tests
Application:	ELISA

Overview
Datasheets

Description

Description: This assay employs an antibody specific for Anti-human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Additional Text

Application Notes

This immunoassay kit allows for the in vitro quantitative determination of Human VEGF concentrations in serum, plasma and other biological fluids. Detection Range: 31.2 - 2000 pg/ml Sensitivity: < 9.0 pg/ml

90-2138 MSDS