CD44 / HCAM Std. Anti-Human and Primate Monoclonal [Clone HCAM/918]

Catalogue Number: 960-MSM2-P1-EQB

Manufacturer:Enquire Bioreagents
Shelf Life:2 years
Preservative:BSA and Azide
Physical state:Liquid
Type:Monoclonal Primary Antibody
Shipping Condition:Blue Ice
Unit(s): 100 ug
Host name: Mouse
Clone: HCAM/918
Isotype: IgG2a, kappa
Immunogen: Recombinant human HCAM protein

Additional Text

Gene Symbol

CD44

Concentration

200ug/ml

Hu-Chromosome Location

11p13

Additional application information

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Molecular Weight

80-95kDa

Specificity

Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.