Catalogue Number: A02050-ABK
Fig.1. Immunofluorescence staining (1:1500) of His fusion protein in 293 cells with red and counterstained with DAPI.
Fig.2. Western blot analysis of 2ug His fusion protein with Anti-His Mouse Monoclonal Antibody (5C3) in 1:5000 dilution (lane A) and 1:10000 dilution (lane B).
Fig.3. IP (1:300) - WB (1:5000) analysis of His fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with His-tag protein (lane B); IP untransfected 293 cell lysate with Anti His tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti His tag mAb (lane E).
|Shelf Life:||12 months|
|Type:||Monoclonal Primary Antibody - Unconjugated|
|Alias:||His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag|
|Shipping Condition:||Blue Ice|
|Unit(s):||200 ul, 5 x 200 ul, 50 ul|
|Application:||IF, IP, WB|
Description: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Featured Anti-His Tag Mouse Monoclonal Antibody (5C3), specially designed for your taged protein quantification and localization.