Sheep anti Human bcl-xl

Catalogue Number: A110P-EXA

Manufacturer:	ExAlpha
Type:	Primary Antibody
Alias:	hbcl-xl, sheep anti human bcl-xl, bclxl, bcl xl, bclx, bcl2L1, BCL2L
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Sheep
Clone:
Isotype:	IgG
Immunogen:	A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence
Application:	WB

Overview

Description

Description: bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs. The larger mRNA gives rise to a protein product, bcl-xl, which is similar in size and predicted structure to bcl-2 (1). The smaller mRNA gives rise to bcl-xS. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2 however membrane bound forms of bcl-x have been demonstrated in thymocytes (2-4). Following the induction of apoptosis all of the bcl-x protein shifts to the membrane form (2). Of the two isoforms of bcl-x, the long (bcl-xl) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression (1,3). Like its homolog bcl-2, bcl-x undergoes phosphorylation, a modification that requires that a specific 60 amino acid loop region be intact, which in turn appears to regulate activity (5,6). Structurally, based on 3D-structure analysis, bcl-x forms pH sensitive cation-selective ion channels in membranes a property shared with the pore forming domains of several bacterial toxins (7). Bcl-xl has been shown to modify the cells response to oxidants, to participate in resistance to chemotherapeutic agents and radiation, and to play a key role in the development of the developing CNS (8-10).

Additional Text

Gene Name

BCL2L1

Gene ID

598

Uniprot ID

Q07817

Purification

Ammonium Sulfate Precipitated

Antibody Clonality

Polyclonal

Application Notes

Application: Western Blot at 1-5 ug/ml. Positive control MCF-7 cells.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Price

£322.00

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