Akt1, PH Domain, Serine-Threonine Phosphorylation (Rac PKa, PKBa) (MaxLight 550)
Catalogue Number: A1125-04-ML550-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 1.T.1 (SKB1) |
| Isotype: | IgG |
| Immunogen: | GST-fusion protein corresponding to residues 1-149 of human Akt1 |
| Application: | FC, ICC, IP, WB |
Description
Description: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. The serine/threonine kinase Akt1 has also been described as Rac-PKa or PKBa. Rac protein kinase family members feature a pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein beta gamma subunits, as well as phosphoinositides. The kinase domain is located between residues 148 to 411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells. Akt1 is a protein of 480 amino acids and has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein. Phosphatase treatment has been shown to result in inactivation of the protein.
Additional Text
Gene Name
AKT1
Gene ID
207
Uniprot ID
P31749
Accession Number
NM_005163
Specificity
Recognizes human Akt1/PKBa > Akt2/PKBb (30%) > Akt3/PKBg (weak to none) based on Western blot analysis using 100ng recombinant fusion protein for each isoform. Species Crossreactivity: mouse and rat.
Purification
Protein G purified
Antibody Clonality
Monoclonal
A1125-04-ML550 Datasheet
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